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Letter to grandmother explaining EAs
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  Playing music in Python
Calling MATLAB functions from Python

Of course, there are several ways to do this. I found the pygame module best for playing MIDI files. Here are some pointers to get started:

After importing pygame,
Set some playback parameters this way:
freq = 44100
bitsize = -16
channels = 2
buffer = 1024    # number of samples
pygame.mixer.init(freq, bitsize, channels, buffer)
pygame.mixer.music.set_volume(1.0)

Now you can try to load and play the file:
pygame.mixer.music.load(music_file) #music_file is the path to your MIDI file
pygame.mixer.music.play()

To stop playing:
pygame.mixer.music.stop()

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